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NAD/NADH and NADP/NADPH are important biological cofactors for oxidoreductase enzymes that are fundamental for various biological processes such as energy metabolism mitochondrial function oxidative stress immunological functions and cell death. Hence the oxidoreductase enzymes are used as indicators of metabolic activity of cells.EZMTT is a novel monosulfonated tetrazolium salt 2-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl) benzenesulfonate sodium salt (EZMTT). The EZMTT reagents provided high quality and reproducible detection for oxidoreductase enzymes indicators of cellular metabolic activity. Upon reacting with metabolic enzymes EZMTT is converted to a yellowish formazan that does not require a solubilization step and can be measured by absorbance at 450 nm. EZMTT assay can continuously track cell proliferation and measure cytotoxicity (loss of viable cells) or cytostatic activity (shift from proliferation to quiescence) of potential medicinal or t
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MW 205.3 Da, Purity >98%. Potent tumor cell proliferation inhibitor in vitro (IC₅₀ = 8 μM). Platelet aggregation inhibitor. Anti-inflammatory and anticancer agent. Active in vitro and in vivo.
Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 100 cells can be accurately quantified. Time efficient. High-throughput assay in 96-well and 384-well plates allows simultaneous processing ten of thousands of samples per day. Homogeneous and convenient. A single reagent and "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: FL530/590nm. Samples: Cell culture. Species: All. Procedure: Assay takes 1-5 hrs, hands-on time 30 min. Kit size: 5000 tests. Detection limit: 100 cells.
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